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Department of Bacteriology, Georgetown University School of Medicine, Washington, D. C.
ABSTRACT
There is a gradual change in the bacterial flora of cheese during the process of ripening. In the hard cheeses, which have not been heated to a high temperature, Streptococcus lactis and Streptococcus cremoris are predominant at first, later being replaced by Lactobacillus casei (Orla-Jensen) Holland (Bacillus casei alpha v. Freudenreich and Thöni, Streptobacterium casei Orla-Jensen), a bacterium which is of importance for the cheese ripening process on account of its proteolytie activity. Many strains of this species show active break-down of casein when inoculated into milk containing calcium carbonate (Orla-Jensen 1919 (1)). No satisfactory explanation for the general occurrence of L. casei has so far been given.1 In the present work a factor for the development of L. casei has been studied, namely the influence of the cell content of Str. lactis and Str. cremori.
EXPERIMENTAL
In the course of the study the following strains were used:
From the collection of Dr. S. Orla-Jensen, Copenhagen: Streptococcus cremoris No. 37, Str. lactis No. 7, Str. lactis No. 17, Str. lactis No. 22, Beta-coccus cremoris No. 7, Streptobacterium casei No. 7, Streptobacterium casei No. 11 and Thermobactermm helveticum No. 12.
1 This organism should not be confused with Lactobacillus helveticus (Orla-Jensen) Bergey et al. {Bacillus casei epsilon v. Freudenreich and Thoni, Thermooacterium helveticum Orla-Jensen) which is of the greatest significance for the ripening of Swiss cheese, but, unlike L. casei, it is not generally found in all kinds of cheeses.
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