HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Salisbury, G. W. Articles by Willett, E. L. Search for Related Content PubMed Articles by Salisbury, G. W. Articles by Willett, E. L.

Preservation of Bovine Spermatozoa in Yolk-Citrate Diluent and Field Results from its Use

Department of Animal Husbandry, Cornell University, Ithaca, N. T.,

E. L. Willett

Seneca Cooperative Cattle Breeders' Association, Inc., Interlaken, N. Y.

ABSTRACT

1. An M/15 solution of sodium citrate mixed in equal amounts with fresh, egg yolk produced a diluent which dispersed the fat globules and other material in the yolk so that when semen was diluted with it the individual spermatozoa could be readily seen upon microscopic examination.
   
2. The yolk-citrate diluent and the yolk-phosphate diluent were apparently equal as preservatives of the motility of spermatozoa which were stored in them tinder standard conditions for two and four days.
   
3. The yolk-citrate diluent was superior to the yolk-phosphate diluent for the preservation of motility when semen was stored six days or more.
   
4. In actual insemination tests with semen stored up to 5 days no significant difference in fertility was found between the semen stored in the yolk-citrate and the yolk-phosphate diluents.
   
5. The results of the fertility studies tend to bear out the results of laboratory storage studies on the maintenance of motility under standard conditions.