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A New Diluent for Bovine Semen

Ohio Agricultural Experiment Station, Wooster

ABSTRACT

A desirable diluent for semen is one that will maintain fecundity of the spermatozoa for many days before it is used for breeding purposes.

Considerable experimental work with diluents versus no diluents for semen has been done in Europe and the United States. The use of egg yolk lecithin in a diluent for semen by Milovanov and Selivanova (2) in Russia, and the use of egg yolk by Phillips and Lardy (4) of Wisconsin has proved helpful in keeping sperm cells viable for some time. The work of Milovanov (2, 3) and Phillips and Lardy (4) suggested an investigation of a combination of gelatin, egg yolk, buffer salts and water, as a diluent material in the artificial insemination studies in progress at the Ohio Agricultural Experiment Station. It is believed that gelatin (Knox) tends to hold sperm inactive, assists in keeping the particles of the egg yolk and the sperm in suspension, supplies extra nutrients, and retards general contamination (bacteria and molds) during storage.