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The Effect of Alfalfa Lipids upon the Progress of Sweet Clover Poisoning in Cattle^*

Departments of Biochemistry and Dairy Husbandry, University of Wisconsin, Madison

ABSTRACT

Ten per cent of the ration of growing cattle was made up of toxic sweet clover and fed without harm for a period of
months.

Animals with a prolonged clotting time developed an increased number of blood platelets. There was no change in the fibrin, hemoglobin, or serum calcium in these cases.

Crude petroleum ether extracts of alfalfa hay fed at a level equivalent to 60 per cent of the toxic sweet clover in the ration brought about a favorable remedial response in sweet clover poisoned young cattle. Evidence adduced from the separate effects upon whole blood clotting time and prothrombin clotting time, the administration of bile salts alone and with alfalfa lipids, and the difference in rate of return to normal between the prothrombin and blood clotting times when the toxic hay was withdrawn from the ration indicates that one or more factors other than prothrombin were involved in the restoration of the normal blood clotting mechanism of the sweet clover poisoned bovine.

^* Published with the approval of the director of the Winsconsin Agricultural Experiment Station.