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The Use of Annatto as a Tracer in Cream for Manufacturing Purposes and its Detection^*

Maryland Agricultural Experiment Station, College Park, Maryland

N. E. Yongue

Health Department, District of Columbia

ABSTRACT

Annatto (vegetable cheese color) is recommended as a universal tracer for use in cream intended for manufacturing purposes. A test for the detection of annatto in cream has been devised. This test is based upon the simultaneous extraction of fat with gasoline and extraction of annatto with alcohol, removal of alcohol-serum by centrifuging, absorption of annatto by an alkaline filter, and confirmation with stannous chloride. The sensitivity of the test is not affected by length of time of storage, freezing, acid production, neutralization, heat, or homogenization. The test is sensitive to 1 part annatto in 500,000 parts of cream.

^* Paper No. 502 in the Scientific Journal Series of the Maryland Agricultural Experiment Station.