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Journal of Dairy Science Vol. 23 No. 4 295-302
© 1940 by American Dairy Science Association ®
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Potentiometric Studies with Resazurin and Methylene Blue in Milk1

C. K. Johns and R. K. Howson

Division of Bacteriology and Dairy Research, Science Service, Department of Agriculture, Ottawa

ABSTRACT

Resazurin reduces to the pink stage in approximately three-fourths of the time required for methylene blue to decolorize. Potentiometric studies indicate that this shortening of reduction time is mainly attributable to the change in shape of the time-potential curve when resazurin is present.

Hourly inversion of tubes during incubation generally shortens the reduction time of good milks considerably. The incorporation of oxygen by this practice has little or no effect upon the time-potential curve during the early stages, and only transient effect later.

FOOTNOTES

1 Contribution No. 59 (Journal Series) from the Division of Bacteriology, Science Service, Department of Agriculture, Ottawa.






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