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Journal of Dairy Science Vol. 22 No. 9 729-735
© 1939 by American Dairy Science Association ®
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Rapid Photoelectric Determination of Carotenoid in Milk Fat

David B. Hand and Paul F. Sharp

Department of Dairy Industry, New York State College of Agriculture, Cornell University, Ithaca, New York

ABSTRACT

Churn 100 ml. samples of cream by shaking; wash the fat with cold water, melt, and centrifuge clear. Transfer 3 ml. to a precision tube absorption cell and warm to about 75° C. to produce a clear solution. Determine the light absorption for blue light in a photoelectric colorimeter and calculate the carotenoid content in mg. per liter of melted fat with the aid of a calibration curve obtained with pure carotene dissolved in cotton seed oil.






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Copyright © 1939 by the American Dairy Science Association ®.