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Journal of Dairy Science Vol. 22 No. 1 7-16
© 1939 by American Dairy Science Association ®
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The Site of Fat Synthesis in the Mammary Gland1

Philip L. Kelly2 and W. E. Petersen

University of Minnesota3

ABSTRACT

  1. An attempt to trace the course of milk fat synthesis by the use of feeds stained with Sudan stain gave negative results partly because of the apparent changing of the structure of the dye molecule to a water soluble compound.
  2. Biochemical studies indicated the presence of quite large amounts of free fatty acids in actively secreting glands. They pointed to a close relationship between the stage of activity and the amount of free fatty acid which was present, with resting glands containing much less of this material than do actively secreting glands. Glands undeveloped by pregnancy contain only a trace of free fatty acid.
  3. Histochemical studies with nile blue sulphate, brilliant cresyl blue, and neutral red indicated the secretory cells of the mammary gland particularly in their basal portions, contained free fatty acids. Neutral fats were located near the distal portions of the alveolus. Oil Red O and osmic acid added confirmatory evidence to the results of the previously mentioned stains.
  4. It is suggested from the evidence presented that the free fatty acids in the basal portions of the secretory epithelium are present in either molecular or colloidal state.

FOOTNOTES

1 A portion of the thesis submitted to the graduate school of the University of Minnesota by the senior author in partial fulfillment of the requirements for the degree of Doctor of Philosophy plus additional material from research at the University of Arkansas Agricultural Experiment Station.

2 Now at the University of Arkansas.

3 Paper No. 1671 Scientific Journal Series, Minnesota Agricultural Experiment Station. Research paper No. 627 Journal Series, University of Arkansas.






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Copyright © 1939 by the American Dairy Science Association ®.