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Note on Violet Red Bile Agar for Detection of Escherichia Coli

Bacteriological Laboratory, Mead Johnson & Co., Evansville, Indiana

ABSTRACT

Since most of the reported work using Violet Red Bile Agar for the detection of Escherichia coli has been on a theoretical laboratory scale, here-with is reported a practical application that yielded such satisfactory results, that it appears worthy of mention.

A dairy, located in one of the North Central States, was having difficulty with a re-contamination by Escherichia coli after the milk received a heat treatment of 170° F. for 30 minutes. The finished product of this dairy could not be marketed unless the material was free from Escherichia coli. It was feared that perhaps the organism was getting into the heated milk from the spray pond water which was used as condensing water in the evaporator and dryer. In an attempt to trace this source of Escherichia coli, the authors were invited as consultants.

Samples of the milk, secured at various points in the process after the milk had been heated as described above, were tested for the presence of Escherichia coli.