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The Resazurin Test—Its Use and Practicability as Applied to the Quality Control of Raw Milk

The Whiting Milk Company, Boston, Mass.

ABSTRACT

An earlier report¹ by Ramsdell, Johnson, and Evans discussed the use of Resazurin as a chemical indicator for determining the sanitary quality of milk. On the basis of the above investigation, it was concluded that the use of Resazurin gave more information as to the quality of milk than any other chemical indicator now in use. Further work on this dye was conducted by C. K. Johns.² Coincident with this latter research, investigations on Resazurin were initiated in these laboratories. It is the purpose of this paper to present data which indicate, first, that the Resazurin test is of added value as an indicator of quality over the Methylene Blue test as well as having the advantage of consuming much less time than the latter test, and second, that the Resazurin test is a valuable adjunct to microscopic diagnosis in routine quality control of milk.

EXPERIMENTAL

Of primary interest was a comparison of the sensitivity of Resazurin to that of Methylene Blue, the standard plate method, and the microscopic Breed smear.

¹ Ramsdell, G. A., Johnson, Jr., W. T., and Evans, F. R. Investigation of Resazurin as an indicator of the sanitary condition of milk. JOUR. DAIRY SCI., 18: 705–717. 1935.

² Johns, C. K. Paper presented at meeting of Vermont Dairy Plant Operators and Managers' Association, November 19, 1936.