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Intra-Mammary Duct Injections in the Study of Lactose Formation^*

College of Agriculture, St. Paul, Minnesota

ABSTRACT

1. Intra-mammary duct injections were found to be a practical way of producing hyperglucemia in the bovine.
   
2. Because the inflow of sugar from the mammary gland apparently stimulates increased insulin activity, sufficient sugar must be injected to exhaust the insulin supply, or hypoglucemia will result.
   
3. In some cases a marked condition of tremors was produced.
   
4. Failure to produce tremors in some of the experiments is thought to be due to a partial dehydration of the animals.
   
5. The onset of a pronounced diuresis accompanied the tremors.
   
6. The diuresis is thought to be more nearly related to the tremors than to the injected sugar.
   
7. It appears that a hyperglucemic condition results in a slight increased lactose secretion.
   
8. The evidence available indicates that colostrum may be an equilibrium product of normally secreted milk rather than a special secretion of the mammary gland.
   

^* The data in this paper are taken mainly from a thesis presented by W. R. Brown in partial fulfillment for the Ph.D. degree and published with the approval of the Director as Paper No. 1379.

¹ From the Division of Agricultural Biochemistry, University of Minnesota.

² From the Division of Dairy Husbandry, University of Minnesota.