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A Comparison of Methods of Detecting Streptococci in Freshly Drawn Milk Samples

Storrs Agricultural Experiment Station, Storrs, Connecticut

ABSTRACT

Conclusions: Microscopic examination of films prepared from incubated milk revealed the presence of the causative organism of chronic streptococcal bovine mastitis in a larger number of instances than (1) direct microscopic examination of films prepared from whole milk or sediment, and (2) blood agar plates inoculated with a 4 mm. loopful of whole milk, or a 4 mm. loopful of sediment, or 1 cc. of a 1 to 10 dilution of the sample.

In the absence of other laboratory evidence of mastitis, the finding of streptococci in incubated samples should not be taken as conclusive evidence of infection with the organism which is commonly responsible for chronic infectious bovine mastitis. The significance of the finding of streptococci in such instances can be determined only by isolation and identification of the streptococcus found in the sample.