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Investigation of Resazurin as an Indicator of the Sanitary Condition of Milk

Research Laboratories, Bureau of Dairy Industry, U. S. Department of Agriculture

ABSTRACT

From the results of this investigation the following conclusions are drawn:

1. Only one hour is required to complete the resazurin test as prescribed in the text, while the methylene blue test requires over five hours.
   
2. Milk can be classified into four groups as regards sanitary condition by means of the resazurin test.
   
3. Milks from diseased udders and milks from physiologically abnormal cows have significant effects on the reduction of resazurin, and hence the test aids in their detection.
   
4. By observing the rate of color change of resazurin-milk mixtures over a period of hours of incubation, considerable information as to the flora can be obtained.