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Department of Dairy Industry, Cornell University, Ithaca, N. Y.
ABSTRACT
The steps involved are saturation with ammonium sulfate, filtering, shaking the nitrate with ethyl ether, separation of the ether layer, neutralization of the acid with alkali, evaporation of the ether, washing of the ethyl ether residue with petroleum ether, taking up the residue with water, heating with sulfuric acid, cooling, and adding guiacol (Denigées test). A red color indicates lactic acid. With care the test is sensitive down to about 0.002 per cent of lactic acid in the milk.
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