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Control of Spreaders in Milk Plates¹

Teachers College, Columbia University, New York

ABSTRACT

Our interest in the problem of the control of spreaders in milk plates was aroused by the occasional difficulties experienced in various milk laboratories. The problem was attacked from the point of the probable effect of differences in humidity in the air inside the agar plates, such as might occur with differences in the concentration of the agar used for the plates. A batch of 1.5 per cent agar was tubed and divided into five parts, which were subjected, respectively, to the various rates of drying which would occur in such places as a laboratory shelf, an incubator, an ice-box, a shelf over a radiator, and a closed tin receptacle, for a period of one week. When this agar was inoculated with spreaders and plated, the driest agar gave most definite evidence of the inhibition of the spreading tendency.

Thereupon, three different percentages of agar were prepared, with a still wider range in the relative agar-water content: 1.5, 2, and 3 per cent, according to the following proportions.

¹ Paper presented at the May meeting of the International Association of Milk Dealers in Washington, 1931.