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Effect of the Electropure Process and of the Holding Method of Treating Milk upon Bacterial Endospores^*

Department of Bacteriology, Michigan State College, East Lansing, Michigan

ABSTRACT

Series of laboratory tests were conducted with some common, spore-forming organisms in order to compare their relative resistance to the Electropure and holding methods of pasteurization. The cultures were from six to eight weeks old when used, and microscopic examinations showed them to contain from 80 to 90 per cent spores. The results obtained in these tests, as shown in table 1 are markedly in favor of the Electropure process. Particular attention is drawn to those results obtained with Bacillus anthracis which is known to be an extremely resistant organism. The percentage reduction in the case of this organism ranged from 0.3 to 2.7 in the holding method, and from 99.5 to 99.7 in the Electropure process. The results of the tests with B. subtilis, B. mycoides, B. mesentericus, and B. megatherium are equally striking.

Concluding from the above results concerning spore destruction the Electropure process at 71°C. with momentary holding proved to be superior to pasteurization at 62.8°C. for 30 minutes, in these laboratory tests.

^* Paper 16 (new series), from Michigan State College Agricultural Experiment Station