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A Study of the Proteolytic Action of Certain Specific Organisms on Milk Proteins in Milk and Synthetic Butter^*

Dairy Department, Purdue University Agricultural Experiment Station, Lafayette, Indiana

ABSTRACT

Considerable work has already been done by a number of investigators on bacterial metabolism including yeasts and molds, with a view of obtaining a quantitative relation of the action of specific organisms when grown in different media. These investigations were generally limited to pathogenic organisms. However, only in a few instances has milk been used as a media. It is evident that in milk we have a natural product, composed of proteins, carbohydrate and mineral salts, well adapted for bacterial growth, the study of which is of prime importance to the dairy industry. It is well known that the composition of the media influences the growth and development of bacteria. For the study of pathogenic organisms the media is usually composed of nitrogenous materials and an adequate supply of mineral salts. It has been found that when these organisms are grown in a media containing some utilizable carbohydrate, that the chemical changes produced in the media are quite different than when grown in media containing no carbohydrate.

^* Approved by the Director of the Agricultural Experiment Station.